2015 ISAKOS Biennial Congress ePoster #415

Assessment of Cell Viability of Fresh Osteochondral Allografts in an Antioxidant Enriched Medium

Rafael Calvo, MD, Santiago CHILE
Maximiliano Espinosa, MD, Santiago, Región Metropolitana CHILE
David Figueroa, MD, Prof., Santiago, RM CHILE
Luz María Pozo, cPhD, Santiago CHILE
José Pablo González, cPhD, Santiago, Metropolitana CHILE
Paulette Conget, PhD, Santiago, RM CHILE

Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Región Metropolitana, CHILE

FDA Status Cleared

Summary: The use of the antioxidant studied at 1 mM concentration prevents a significant decrease in cell viability in chondrocytes obtained from human osteochondral tissue. However higher concentrations do not prevent this fall.

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Abstract:

Objective

To evaluate the effect of an antoxidant enriched storage medium on fresh osteochondral viability at 4 ° C

Hypothesis

Cell viability of chondrocytes obtained from human osteochondral tissue and stored at 4 ° C improves significantly in the presence of the antioxidant.

Materials And Methods

Experimental study in 9 samples of femoral condyle osteochondral tissue. Samples were obtained from patients undergoing total knee replacement. The samples were kept stored at 4 ° C in PBS or three concentrations of an antioxidant (AO 1,2,5mM) and disrupted enzymatically at time 0 and 4 weeks. Cell viability was analyzed by flow cytometry. The results of cell viability (median) were analyzed statistically using non parametric Kruskall Wallis test. P values <0.05 were considered statistically significant.

Results

The viability at time 0 was considered 100%. At 4 weeks, the cell viability in the control group (PBS) was 35.6%, in the group stored with AO 1 mM was 76.9%, in the group stored with 2mM AO was 50% and in the group stored with AO 5 mM was 25%. A statistically significant difference from baseline viability (time 0) was observed in PBS control group (p = 0.0481), 2 mM AO (0.0442) and 5 mM AO (0.005). However, in the group stored with 1 mM AO, no statistically significant difference with the initial viability was observed (p = 0.605).

Conclusions

The use of the antioxidant studied at 1 mM concentration prevents a significant decrease in cell viability in chondrocytes obtained from human osteochondral tissue. However higher concentrations do not prevent this fall.

Clinical Relevance: The enrichment of the storage medium of fresh osteochondral allografts with an antioxidant, could increase the storage time of these and thus the time window for its use in patients.