2015 ISAKOS Biennial Congress ePoster #102

Transplantation of Adipose-Derived Mesenchymal Stem Cells for Ankle Articular Cartilage Focal Lesions

Michail I. Iosifidis, MD, PhD, Thessaloniki GREECE
Tryfon Totlis, MD, PhD, Edessa GREECE
Konstantinos Apostolidis, MD, Thessaloniki GREECE
Theofylaktos Kyriakidis, MD, MSc, PhD, Brussels BELGIUM
Efstathios Michalopoulos, PhD, Athens GREECE
Theophanis Chatzistamatiou, PhD, Athens GREECE
Andreas Papasavas, PhD, Athens GREECE
Ioannis Tsitouridis, MD, PhD, Thessaloniki GREECE
Aikaterini Stavropoulos, MD, PhD, Athens GREECE
Anastasios Miltiades Kyriakidis, Senior Consultant, Thessaloniki GREECE

2nd Orthopaedic Department, "Papageorgiou" General Hospital, Thessaloniki, Radiology Department, "Papageorgiou" G.H., Thessaloniki, Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens (BRFAA), Thessaloniki, Athens, GREECE

FDA Status Not Applicable

Summary: The use of MSCs for ankle focal cartilage lesions, a safe and promising procedure





of this pilot prospective study was to evaluate the results of transplantation of adipose-derived mesenchymal stem cells for the treatment of focal ankle chondral lesions.

Matelrial and Methods: Six patients (male:female 2:4), mean aged 28.67 (18-42 years), having 6 ICRS grade 3 and 4 focal ankle chondral defects were included. Medial malleolar osteotomy was performed in four patients having a posteromedial talar lesion. One patient with an anteromedial talar lesion and another having a tibial plafond lesion underwent an all-arthroscopic procedure. This single-staged procedure included filling of each defect with autologous culture-expanded mesenchymal stem cells, isolated from abdominal subcutaneous fat and embedded in a trimmed-to-fit commercially available biodegradable matrix. Patients were evaluated using the Foot and Ankle Outcome Score (FAOS) scale at 1st, 3rd, 6th, 12th and 24th p.o. month (mean 17.5 months). The non-parametric Wilcox Signed Ramk test was used to determine significant differences between preoperative and postoperative values (p<0.05). In addition the patients underwent MRI (3 Tesla) at the 12th and 24th p.o. month using the Magnetic Resonance Obsrevation of Cartilage Repair (MOCART) scoring system.


No complications or adverse events were reported. All six patients were found to be satisfied. A significant increase in mean values of all FAOS subscales was found, whereas the total FAOS improved from 32.8 to 78.6, p=0.05. The MOCART score revealed significant development of tissue resembling the healthy cartilage.


Treatment of focal ankle chondral lesions with this single-staged cell-based technique demonstrated promising preliminary results, according to relevant outcome scores. It is need longer follow up period and larger patients number in order to reach safer results for the effectiveness of this treatment option.