2015 ISAKOS Biennial Congress ePoster #407

Treatment Outcomes of Fibrin Matrix Allogenic Chondrocyte Implantation and Autologous Bone Marrow Cell Stimulation for the Repair of Full Thickness Articular Cartilage Defects in a Rabbit Model

Chen Tai Teong, MD, Jeju KOREA, REPUBLIC OF
Sung-Wook Choi, MD, PhD, Prof., Jeju KOREA, REPUBLIC OF
Jeju National University, Jeju, KOREA

FDA Status Cleared

Summary: The experimental results of this study suggest that the implantation of gel-type fibrin matrix with allogenic chondrocytes and autologous bone marrow cells may be a useful methodology to regenerate hyaline-like cartilage in full-thickness cartilage defect

Abstract:

Introduction

We evaluated the results of allogenic chondrocyte implantation (AlloCI) and autologous bone marrow cell stimulation (AutoBMS) using a 3-dimensional fibrin matrix to promote differentiation and increase mechanical stability of implanted chondrocytes.

Method:Eighteen rabbits were divided into two groups. One group was treated with a mixture of allogenic chondrocytes and fibrin matrix to the articular cartilage defect (AlloCI:n=9), and the other group with placement of microfracture to the cartilage defect and covering it with fibrin (AutoBMS:n=9). A control was set for each object with untreated opposite knee. The rabbits were sacrificed at 6 months after treatment and then cartilage tissue was evaluated with the International Cartilage Repair Society(ICRS) scoring system, immunohistochemical staining and modified O'Driscoll grading system.

Results:The ICRS scores were very similar in the AlloCI(9.33 ± 1.87) and theAutoBMS(9.44 ± 2.33). Immunohistochemical staining for type II collagen further confirmed that the tissue section from both autoBMS- and alloCI-treated animals showed a relatively higher expression of cartilaginous collagen compared with those of the control groups. The average difference (AlloCI, 32.89 ± 5.25 ; AutoBMS, 31.89±6.45) of the modified O’Driscoll scores between the treatment groups appeared to be very small and nonsignificant (P>0.05), whereas, both treatment groups showed significantly higher modified O’Driscoll scores compared to those of their respective controls (19.77±1.58 ; 18.95±2.05)(P<0.05).

Conclusion:The experimental results of this study suggest that the implantation of gel-type fibrin matrix with allogenic chondrocytes and autologous bone marrow cells may be a useful methodology to regenerate hyaline-like cartilage in full-thickness cartilage defect.


Key words: allogenic chondrocyte, autologous bone marrow cell stimulation, cartilage repair