2017 ISAKOS Biennial Congress ePoster #232

 

Hyaline Cartilage Regeneration on Osteochondral Defects of Knee Joints with Intraarticular Human Peripheral Blood CD34+ Stem Cells, Hyaluronic Acid, TGF, IGF, FGF and Fibronectin Injection In Sprague Dawley Rats

Basuki Supartono, MD, FICS, MHA, PhD, East Jakarta, DKI Jakarta INDONESIA
National Sports Hospital of Indonesia, University of Pembangunan Nasional " Veteran" Jakarta, Jakarta, DKI Jakarta, INDONESIA

FDA Status Not Applicable

Summary

CD34+ Regenerate Hyaline Cartilage in Osteochondral Defect of Knee Joint

Abstract

The knee joint cartilage often suffers from defect and causes serious health problem. An adult human circulating CD34+ cells have been demonstrated to contribute for fracture healing, but its capability still unknown in hyaline cartilage regeneration. This is the first study reporting hyaline cartilage regeneration on osteochondral defect with intraarticular injection of CD34+ cell. The defect was created on the trochlear region of Sprague Dawley (SD) rats. A total of 30 male SD rats were random divided into 3 groups; the control group received PBS, experimental group 1 received CD34+ cells, and experimental group 2 received CD34+ and growth factors/scaffold (hyaluronic acid, TGF-ß1, IGF-1, FGF, fibronectin). Laboratory, radiology, macroscopic and microscopic evaluations were done on week 4th and 8th. At week 4th and 8th, both experimental groups showed the superficial defects fully filled with hyaline cartilage but not in control group, suggesting that CD34+ cells promoted cartilage regeneration. In conclusion, human peripheral blood CD34+ stem cells can promotes hyaline cartilage regeneration of the knee joint osteochondral defect on SD rats.