2017 ISAKOS Biennial Congress ePoster #1135

 

Contaminated Anterior Cruciate Ligament Graft: Effect On Maximum Tensile Force After Being Rinsed With Gentamicin Or Clorhexidine.

Maximiliano Andres Barahona, MD, MSc, Santiago, RM CHILE
Miguel J. L. Palet, MD, Santiago CHILE
Alvaro Igor Zamorano Cadenas, MD, Santiago, RM CHILE
Jaime Alejandro Catalán, MD, Santiago CHILE
Carlos A. Infante, MD, Santiago CHILE
Jaime Hinzpeter, MD, Santiago, RM CHILE

Hospital Clinico Universidad de Chile, santiago, Region Metropolitana, CHILE

FDA Status Cleared

Summary

FIRST EVIDENCE ON THE EFFECT ON MAXIMUM TENSILE FORCE AFTER BEING RINSED WITH GENTAMICIN OR CLORHEXIDINE.

Abstract

Background

Anterior cruciate ligament (ACL) contamination is rare, but it is a risk for developing septic arthritis. Chlorhexidine and Gentamicin were found to be effective sterilization agents, nevertheless there is no biomechanical studies evaluating sterilized grafts after being rinsed. Aim: To compare the maximum tensile force (MTF) of semitendinosus (ST) and gracilis (G) after being rinsed with Chlorhexidine or Gentamicin. Material and Methods: Experimental Study. They were recruited 10 consecutive patients with ACL injury, reconstructed with ST- G technique. After the tibial fixation, the excess graft was cut with a scalpel, and a tail of each tendon was washed for 8 minutes in a solution containing 2% Chlorhexidine and the other pair in physiological serum + 80mg Gentamicin. The MTF was tested on a universal tensiometer Tinius-S Olsen® H5K, at a speed of 5 mm per minute. Prior to testing, the graft diameter was measured with a digital micrometer. The data are presented in median and interquartile range, nonparametric median test for unpaired data was performed to compare MTF per diameter of graft. Stata v11.2 (StataCorp LP, College Station, Texas, USA) was used. Results: 30 graft tails were analyzed, 14 G and 16 ST. The median MTF per diameter for Chlorhexidine group was 91N/mm [80-101] and gentamicin group was 86N/mm [70-103], the difference did not achieved significant difference (p = 0.36). Conclusions: No differences between in MTF after the rinse with Chlorhexidine or Gentamicin was found, therefore both options are valid after contamination graft.