2017 ISAKOS Biennial Congress ePoster #312
The Use Of Silk Fibroin Coating To Enhance Intra-Articular Ligamentization Of Polyethylene Terepthalate Artificial Ligament
Chengchong Ai, MD, Shanghai CHINA
Jia Jiang, MD, PhD, Shanghai CHINA
Fang Wan, MD, PhD, Shanghai CHINA
Shiyi Chen, MD, PhD, Prof., Shanghai CHINA
Huashan Hospital, Fudan University, Shanghai, Shanghai, CHINA
FDA Status Not Applicable
Summary
Our results in vitro demonstrated SF coating enhanced proliferation, adhesion and regular orientation of fibroblast cells, which was consolidated in promoting intra-articular ligamentization in vivo. The overall finding indicated that SF coating could effectively promote the self-tissue ingrowth into the PET synthesis using in the ACL reconstruction.
Abstract
Background
In recent years, Ligament Advanced Reinforcement System(LARS) was back in the spotlight due to its positive clinical outcome, but synovitis remains concerning. Silk fibroin (SF), which is characterized by good biocompatibility and low immunogenicity, has shown its versatility as a novel material and has been attempted in tissue engineering for its slow biodegradation and good mechanical properties required by bone, ligament and musculoskeletal tissues. We focused on the ligamentization of intra-articular segment of graft in this study and hypothesized that the graft coated SF would show better biocompatibility and ligamentization process.
Methods
A PET artificial ligament with SF coating was prepared via plasma pretreatment, then by SF dipping process.Scanning electron microscopy (SEM) and atomic force microscope (AFM) were utilized for proving the introduction of SF and characterizing the changes in surface properties. The performance of SF-coated PET graft (PET+SF) and non-coated PET graft (PET) were compared in vitro by culturing fibroblast cells and analyzed by SEM, confocal microscopy, CCK-8 assay and the expression level of integrinß1 detection. Furtherly, PET+SF ligaments and PET ligaments were respectively adopted in beagle model to reconstruct the anterior cruciate ligament (ACL).The intra-articular segment was evaluated by histological and immunohistochemical tests 4 and 8 weeks postoperatively.
Results
The SF was successfully coated on the surface of PET fiber. The SEM, confocal microscopy and CCK-8 assay showed that the fibroblast cell proliferation on PET+SF group was better than the PET group. After 3 days of culture, the expres¬sion level of integrin ß1 in the PET+SF group and was higher than in the PET group. The in vivo results showed a thicker tissue enclosed the free fiber in articular cavity . Masson staining showed that in the PET+SF group 8 weeks postoperatively, the PET fibers were almost completely encircled by collagen. In addition, the expressing level of collagen I in the PET+SF group was higher than in the PET group by immunohistochemical analysis.