2017 ISAKOS Biennial Congress IFOSMA ePoster #5033

 

Osteochondral Regeneration Induced by TGF-Loaded Photo Cross-linked Hyaluronic Acid Hydrogel Infiltrated in Fused Deposition Manufactured Composite Scaffold of Hydroxyapatite and Poly (ethylene glycol)-block-Poly(-caprolactone)

Yi-Ho Hsieh, Taiwan, Taiwan CHINA
Ming-Fa Hsieh, Taiwan, Taiwan CHINA

Chung Yuan Christian University,, taiwan, taiwan, china

FDA Status Not Applicable

Summary

Osteochondral Regeneration Induced by TGF-Loaded Photo Cross-linked Hyaluronic Acid Hydrogel Infiltrated in Fused Deposition Manufactured Composite Scaffold of Hydroxyapatite and Poly (ethylene glycol)-block-Poly(-caprolactone)

Abstract

Introduction

Osteoarthritis is a musculoskeletal disease prevailed in the elderly, and can hamper the physical mobility in daily life. Tissue engineering approach has been employed to regenerate cartilage and bone as well. Recently rapid prototyping has emerged to fabricate scaffolds with desired internal architecture as physical cues to harbor both differentiated cells and stem cells. As such, this study reports the fabrication of porous scaffolds with pre-designed internal pores using fused deposition modeling (FDM)

Methods

The filaments of methoxy poly(ethylene glycol)-block-poly(ε-caprolactone) having terminal groups of carboxylic acid were deposited layer by layer. Raw materials having a weight ratio of hydroxyapatite (HAp) to polymer of 1:2 was used for FDM. The scaffolds with three different orientations of the filaments were fabricated e.g. 90o-, 45o-interlaced, and parallel shift filaments between two layers. HAp was found to significantly increase the compressive strength of the porous scaffolds. Among three orientations of the filaments, the 90o-interlaced scaffolds exhibited highest compressive strength. To promote cell adhesion, amino groups of the Arg-Gly-Asp peptide were condensed with the carboxylic groups on the surface of the fabricated scaffold. Bone marrow-derived stem cells seeded in the scaffolds differentiated into chondrocytes and maintained characteristic phenotypes of collagen type 2, aggrecan and SOX-9 in a time-dependent manner. A second phase of hydrogel of glycidyl methacrylate hyaluronic acid loading with TGF- was photo cross-linked on the top of the scaffolds implanted in the osteochondral wound of rabbit knee.
Result:
Histological sections indicated that the subchondral bone and cartilage were fully healed 12 weeks post the implantation of the TGF- loaded hydrogel and scaffolds, and regenerated cartilage was hyaline cartilage as indicated by alcian blue and periodic acid-schiff double staining.

Conclusion

In our study, we showed rapid prototyping scaffold with light-curing gel containing factor, that could enhance osteochondral defect healing. Defect with good integration, coupled to hyaline cartilage repair tissue without damaging the adjacent native articular cartilage. The bio-composite implanted into osteochondral drilling hole, blood with mesenchymal stem cells mixed to the implanting by capillarity, differentiating into chondrocytes on the implanting. Compared to current treatment of osteoarthritis, our study showed a new method, clinical option and development of osteoarthritis.