2017 ISAKOS Biennial Congress ePoster #2236

 

Effect of bFGF-Loaded Ca5(Po4)2Sio4 Bioceramic on Tendon-To-Bone Healing After Repair of Chronic Rotator Cuff Tear

Song Zhao, MD, Shanghai CHINA
Guoming Xie, PhD, Shanghai CHINA
Xiaoqiao Huangfu, MD, Shanghai CHINA
Xiaoqiao Huangfu, MD, Shanghai CHINA
Jinzhong Zhao, MD, Shanghai CHINA

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, CHINA

FDA Status Not Applicable

Summary

We investigated whether augmented repairs with BFGF-loaded CPS bioceramic could aid the reconstruction of the tendon-bone insertion and will enhance rotator cuff healing

ePosters will be available shortly before Congress

Abstract

Background

Tendon-to-bone healing after rotator cuff (RC) repair depends on bone ingrowth into the granulation tissue at the tendon-bone interface. A novel calcium phosphate-based biomaterial (CaP), Ca5(PO4)2SiO4 (CPS) bioceramic, exhibited better bioactivity, cytocompatibility and osteogenic activity in vitro. We hypothesize that augmented repairs with BFGF-loaded CPS bioceramic will aid the reconstruction of the tendon-bone insertion and will enhance tendon-to-bone healing in vivo.

Methods

One hundred forty-four male Sprague-Dawley rats underwent unilateral detachment of the supraspinatus tendon, followed by delayed repair after three weeks. The animals were allocated into one of three groups: (1) repair alone, (2) repair augmented with BFGF-loaded CPS bioceramic, or (3) repair augmented with hydroxyapatite (HA) bioceramic at the tendon-bone interface. Animals were killed at 2, 4, or 8 weeks postoperatively. Microcomputed tomography was utilized to quantify the new bone formation at the repair site. New fibrocartilage formation and collagen organization at the tendon-bone interface was evaluated by histomorphometric analysis. Biomechanical testing of the supraspinatus tendon-bone complex was performed. Statistical analysis was performed using one-way analysis of variance. Significance was set at p<0.05.

Results

Microcomputed tomography analysis demonstrated remarkable osteogenic activity and osteoconductivity to promote new bone formation and ingrowth of BFGF-loaded CPS bioceramic. Histological observations indicated that BFGF-loaded CPS bioceramic had excellent biocompatibility and biodegradability. At early time points after the RC repair, BFGF-loaded CPS bioceramic significantly increased the area of fibrocartilage at the tendon-bone interface compared with the control and HA groups. Moreover, BFGF-loaded CPS and HA bioceramics had significantly improved collagen organization. Biomechanical tests indicated that the BFGF-loaded CPS and HA groups have greater ultimate load to failure and stiffness than the control group at 4 and 8 weeks, and the BFGF-loaded CPS specimens exhibited the maximum ultimate load to failure, stiffness, and stress of the healing enthesis.

Conclusions

CaP bioceramics aid in cell attachment and proliferation and accelerate new bone formation, and BFGF-loaded CPS bioceramic has a more prominent effect on tendon-to-bone healing.