2015 ISAKOS Biennial Congress ePoster #413

An Examination of Growth Factor Levels in Platelet-Rich Plasma and Correlations with Age, Gender, Platelet Count, and DHEAS

Aki Osawa, MD, PhD, Urayasu, CHIBA JAPAN
Yuichiro Maruyama, MD, PhD, Urayasu JAPAN
Kazuo Kaneko, MD, PhD, Prof., Tokyo JAPAN

JUNTENDO UNIVERSITY URAYASU HOSPITAL, URAYASU, CHIBA, JAPAN

FDA Status Not Applicable

Summary: PRP contains a number of growth factors for the repair of tissue. It is remains unclear the correlation between growth factor and individual differences. The aim of this study is to analyze the correlation between varying factors: age, gender, platelet count and DHEAS with growth factor level. This study shows correlations between varying growth factors and individual differences.

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Abstract:

Introduction

Platelet-Rich Plasma (PRP) is used as an injective treatment or as a surgical augmentation procedure for the repair of tissue with low healing potential. PRP contains a number of different growth factors such as TGFb1, VEGF, EGF, and PDGF. It is clear that PRP has a positive role in tissue repair. However, up to now, there have been only a few reports examining the correlation between growth factor and individual differences. The aim of this study is to analyze the correlation between varying factors: age, gender, and platelet count with growth factor level for the purpose of healing potential. This study also introduces a fourth difference, DHEAS, which is a steroid hormone and is used as an aging marker to show its correlation with growth factor level.
MATERIALS AND METHODS:This study was approved by the Institinal Review Board and the local Ethics Commmittee. Thirty-two healthy volunteers were enrolled to provide a blood sample. These subjects included 12 males and 20 females within an age range of 19 to 59(34.4?10.2) The collected blood was then drawn from the collection tube into an Arthrex ACP (PRP) double syringe system (Arthrex systems, Naples, Florida) and spun at 1500 rpm for 5 minutes in a centrifuge (Hettich Rotofix 32, Tuttlingen, Germany) as described by the standard manufacturer protocol. Aliquots of whole blood were measured for platelet concentrations and DHEAS. Four growth factors were analyzed in this study using enzyme-linked immunosorbant assay kits specific for each growth factor (R & D systems). TGFb1, VEGF, EGF and PDGF were measured through the manufacturer’s recommendations. All standards and samples were run in duplicate. A Mann-Whitney rank-sum test was conducted to examine the relationship with each growth factor. Pearson’s correlations coefficients were used to test the correlation between age, platelet count, DHEAS and each of the four growth factors.
RESULT: In this study, the tested samples of male and female subjects showed a significant difference in terms of TGFb1 (p<0.05). In terms of age, younger subjects displayed an increased TGFB and PDGF in comparison to elder subjects. DHEAS was correlated with PDGF and PLT was correlated with VEGF.

Discussion

This study shows correlations between varying growth factors and individual differences. However, as the sample group was relatively small, more research needs to be done to confirm these findings. Furthermore, there remains a need to examine the healing potential in vivo.