2019 ISAKOS Biennial Congress ePoster #635
Comparison of Self-Renewal and Differentiation Potential for Synovial Mesenchymal Stem Cells between Non-Arthritis and Osteoarthritis in the Hip Joint
Yoichi Murata, MD, Kitakyushu, Fukuoka JAPAN
Hajime Utsunomiya, MD, PhD, Tokyo , Tokyo JAPAN
Akihisa Hatakeyama, MD, Kitakyushu, Fukuoka JAPAN
Hirotaka Nakashima, MD, PhD, Kitakyusyu, Fukuoka JAPAN
Toshiharu Mori, MD, PhD, Kitakyushu, Japan JAPAN
Ichiro Sekiya, MD, PhD, Bunkyo-Ku, Tokyo JAPAN
Akinori Sakai, MD, PhD, Kitakyushu, Fukuoka JAPAN
Soshi Uchida, MD, PhD, Kitakyushu, Fukuoka JAPAN
Wakamatsu Hospital of University of Occupational and Environmental Health, Department of Orthopaedic Surgery and Sports Medicine, Kitakyushu, Fukuoka, JAPAN
FDA Status Not Applicable
Summary
The OA synovium derived MSCs had greater adipogenic and osteogenic differentiation potential than non-arthritis synovium derived MSCs.
Abstract
Background
Recent studies have shown that patients with advanced osteoarthritis (OA) or severe cartilage damage do not benefit from hip arthroscopic labral repair and correction surgery. Understanding the characteristics of mesenchymal stem cells (MSCs) in non-arthritis or OA of the hip could give an insight into etiology and stem cell treatment options.
Purpose
To isolate and define the characteristics of MSCs obtained from synovium of the hip joints of patients with non-arthritis and OA, and to identify whether there are any differences between synovial MSCs from non-arthritis and OA.
Study Design: Controlled laboratory study.
Methods
Synovium was harvested from the patients with both non-arthritis (femoroacetabular impingement) (21 donors) and OA (14 donors). The number of cells, colony forming units, viability and differentiation capabilities of adipose, bone and cartilage were collected and compared between both groups.
Results
The number of colonies at passage 0 from the OA synovium was significantly higher than from the non-arthritis synovium (P< .01), however, expandability and viability of cells from OA synovium was lower than those from non-arthritis synovium. Based on adipogenesis experiments, expression of PPARG in cells from OA synovium was higher than those from the non-arthritis synovium (P < .05). Based on osteogenesis experiments, the frequency of von Kossa and alkaline phosphatase positive colonies and the expression of COL1a1, ALPP, RUNX2 and BGLAP in cells from OA synovium was higher than those from the non-arthritis synovium (P < .05). Based on chondrogenic experiments, the expression of COL2a1 in cells from OA synovium tends to higher than those from non-arthritis synovium. On the other hand, the expression of COL10a1 in cells from OA synovium was lower than those from non-arthritis synovium (P < .05).
Conclusion
This study demonstrates that synovial MSCs harvested from the hip joints of patients with non-arthritis and OA have different self-renewal and multi-lineage differentiation potential. The colony number per nucleated cell number from the OA synovium was higher than that from the non-arthritis synovium. The OA synovium derived MSCs had greater adipogenic and osteogenic differentiation potential than non-arthritis synovium derived MSCs.